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ATAC-Seq Library Preparation of Murine Bone Marrow-Derived Neutrophils
Published on: January 3, 2025
Neutrophil-Mediated Inflammatory Response to Zinc Bone Implants: A Single-Cell Transcriptomic Landscape
Mi Du1,2,3,4, Qinyang Yan5,6, Chen Zhu5,6
1Department of Implantology, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University, Jinan, China.
Advanced Healthcare Materials
|July 14, 2026
Summary
Zinc implants promote bone repair by altering immune cell responses. Zn-based metals modulate neutrophil and macrophage interactions, enhancing tissue regeneration compared to titanium implants.
Area of Science:
- Biomaterials Science
- Immunology
- Regenerative Medicine
Background:
- Zinc (Zn)-based metals are promising for bone regeneration.
- Neutrophils are key in early inflammation and resolution.
- Mechanisms of Zn-implant-induced inflammation require further study.
Purpose of the Study:
- Investigate neutrophil-macrophage crosstalk around Zn-based implants.
- Compare immune responses to Zn vs. titanium (Ti) implants.
- Elucidate Zn implant's role in immune cell modulation.
Main Methods:
- Single-cell transcriptomic profiling in a rat femoral model.
- Generation of a cellular atlas of the peri-implant bone marrow microenvironment.
- Analysis of neutrophil and macrophage subset heterogeneity and crosstalk.
Main Results:
- Zn implants induced distinct neutrophil subsets, including Prtn3+ Neu with enhanced tissue repair potential.
- Zn implants reduced macrophage reactivity, promoting Hp+ Mac dominance over Ti's Apoe+ Mac.
- Identified Lgals9-Ighm axis mediating Ifit1bl+ Neu and Eno3+ Mac interaction under Zn.
Conclusions:
- Degradation products of Zn-based implants modulate immune cell differentiation and interactions.
- Zn implants influence immune cell networks, impacting biological response for bone regeneration.
- Zn-based metals offer a distinct immunomodulatory profile for bone repair applications.
