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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...

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Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors
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Enzyme-Activatable Fluorogenic Probes: Design Strategies, Biomedical Applications, and Future Perspectives.

Yufan Fan1, LeLe Liu2, Hairong Zeng1

  • 1State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine, Shanghai Frontiers Science Center of TCM Chemical Biology, Institute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.

Journal of the American Chemical Society
|July 14, 2026
PubMed
Summary

Enzyme-activatable fluorogenic probes (EAFPs) offer advanced tools for monitoring enzyme activity in real-time. These probes are crucial for understanding disease mechanisms and developing new diagnostics and therapeutics.

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Area of Science:

  • Biochemistry and Molecular Biology
  • Chemical Biology
  • Biomedical Engineering

Background:

  • Enzymes are critical for cellular functions, and their dysregulation is linked to various diseases.
  • Accurate monitoring of enzyme activity is vital for understanding biological processes and disease pathology.
  • Enzyme-activatable fluorogenic probes (EAFPs) have emerged as powerful tools for real-time enzyme activity assessment.

Purpose of the Study:

  • To provide a comprehensive overview of the state-of-the-art enzyme-activatable fluorogenic probes (EAFPs).
  • To discuss the fundamental design principles, molecular engineering, and sensing mechanisms of EAFPs.
  • To highlight the diverse biomedical applications and future potential of EAFPs in research and clinical settings.

Main Methods:

  • Review and synthesis of current literature on EAFPs.
  • Analysis of molecular engineering, sensing mechanisms, and design strategies for EAFPs.
  • Presentation of representative examples of EAFPs in various biomedical applications.

Main Results:

  • EAFPs have evolved significantly, offering high spatiotemporal resolution and multifunctional biosensing capabilities.
  • Cutting-edge EAFPs demonstrate diverse structural features and recognition mechanisms for specific enzyme targets.
  • EAFPs show significant utility in biomarker imaging, disease diagnosis, drug screening, and therapeutic testing.

Conclusions:

  • EAFPs are indispensable tools for studying enzyme dynamics and disease mechanisms.
  • Continued development of high-performance EAFPs will accelerate fundamental research and translational applications.
  • Future research should focus on addressing existing challenges to further enhance EAFP capabilities for specific scenarios.