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Recent advances in microbial laccases for aflatoxins control: Identification, modification, and application.
Xin Zhang1, Binbin Ouyang2, Wei Xu2
1College of Food and Bioengineering, Zhengzhou University of Light Industry, 136 Kexue Avenue, Zhengzhou, 450000, PR China.
Microbial laccases can transform harmful aflatoxins (AFs), protecting food security. Research reviews their properties, applications, and advancements in protein engineering and immobilization for effective AFs control.
Area of Science:
- Biochemistry
- Enzymology
- Food Science
Background:
- Aflatoxins (AFs) are potent carcinogens threatening global food and feed security.
- Microbial laccases are copper-containing oxidoreductases extensively studied for aflatoxin transformation.
- Laccases offer environmentally friendly properties and diverse applications.
Purpose of the Study:
- To systematically compare enzymatic properties of aflatoxin-transforming laccases.
- To review applications of laccases in aflatoxin control.
- To discuss recent advances in molecular modification and enzyme immobilization.
Main Methods:
- Literature review of microbial laccases capable of aflatoxin transformation.
- Analysis of crystal structures to propose catalytic mechanisms.
- Evaluation of protein engineering and enzyme immobilization strategies.
Main Results:
- Numerous microbial laccases with aflatoxin-transforming capacity have been identified.
- Crystal structures of two laccase types have been resolved, aiding mechanism elucidation.
- Progress in protein engineering and immobilization enhances enzyme efficiency and reusability.
Conclusions:
- Microbial laccases are promising biocatalysts for aflatoxin detoxification.
- Further research in enzyme engineering and immobilization will improve practical applications.
- Laccase-based strategies offer sustainable solutions for aflatoxin contamination control.
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