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Enhanced Fluorescent Aptasensor Via Tetrahedral DNA Framework-Mediated Multivalent Aptamer Network and
Luyu Wei1, Mingrui Xu1, Yinjiao Shi1
1The Modernization Engineering Technology Research Center of Ethnic Minority Medicine of Hubei Province, School of Pharmaceutical Sciences, South-Central Minzu University, Wuhan 430074, China.
Analytical Chemistry
|July 14, 2026
Summary
This study presents an enhanced fluorescent aptasensor using a multivalent aptamer network and carbon quantum dots for sensitive detection. The novel design significantly improves target recognition and enables trace-level detection of aflatoxin B1.
Area of Science:
- Biotechnology
- Nanotechnology
- Analytical Chemistry
Background:
- Fluorescent aptasensors offer high specificity and rapid response for molecular diagnostics.
- Sensitivity limitations arise from low aptamer recognition and suboptimal fluorescent reporter properties.
Purpose of the Study:
- To develop an enhanced fluorescent aptasensor with improved sensitivity and recognition efficiency.
- To address the limitations of traditional aptasensors for trace-level detection.
Main Methods:
- Constructed a multivalent aptamer network (MAN) using a reverse tetrahedral DNA (RTDNA) scaffold and magnetic nanoparticles.
- Engineered high-quantum-yield carbon quantum dots (CQDs) via a multiobjective optimization strategy.
- Integrated MAN and CQDs for a "fluorescence-on" signal generation through DNA polymerization.
Main Results:
- The MAN architecture enhanced aptamer affinity by approximately 3-fold compared to monovalent aptamers.
- The developed aptasensor achieved a linear range of 10^2-10^6 pg/mL for aflatoxin B1 detection.
- A low detection limit of 23.96 pg/mL for aflatoxin B1 was demonstrated, with reliable performance in complex food matrices.
Conclusions:
- The enhanced fluorescent aptasensor demonstrates high sensitivity and reliability for trace-level detection.
- The combination of MAN and CQDs offers a promising strategy for advanced molecular diagnostics.
- This platform shows significant potential for application in food safety and contaminant analysis.
