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Related Concept Videos

Metastasis02:30

Metastasis

Metastasis is the spread of cancer cells from the original site to distant locations in the body. Cancer cells can spread via blood vessels (hematogenous) as well as lymph vessels in the body.
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...

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Related Experiment Video

Updated: Jul 16, 2026

A Robust Discovery Platform for the Identification of Novel Mediators of Melanoma Metastasis
07:41

A Robust Discovery Platform for the Identification of Novel Mediators of Melanoma Metastasis

Published on: March 8, 2022

Characterizing the Mutational Landscape of In-Transit Melanoma Metastases.

Luisa Quesada Camacho1, Mohamad El Moheb2,3, Mackenzie M Mayhew2

  • 1Department of Biomedical Engineering, University of Virginia, Charlottesville, Virginia, USA.

Pigment Cell & Melanoma Research
|July 15, 2026
PubMed
Summary

In-transit melanoma (ITM) shows a distinct mutational profile, enriched for NRAS Q61 mutations. This finding may explain ITM

Keywords:
cutaneous melanomagenomic profilingin‐transit melanomamelanoma metastasesmutational landscape

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Last Updated: Jul 16, 2026

A Robust Discovery Platform for the Identification of Novel Mediators of Melanoma Metastasis
07:41

A Robust Discovery Platform for the Identification of Novel Mediators of Melanoma Metastasis

Published on: March 8, 2022

A 3D Organotypic Melanoma Spheroid Skin Model
08:49

A 3D Organotypic Melanoma Spheroid Skin Model

Published on: May 18, 2018

Experimental Metastasis Assay
08:28

Experimental Metastasis Assay

Published on: August 24, 2010

Area of Science:

  • Oncology
  • Genetics
  • Dermatology

Background:

  • In-transit melanoma (ITM) presents unique therapeutic challenges.
  • The molecular drivers of ITM are not well understood.

Purpose of the Study:

  • Characterize the mutational landscape of ITM.
  • Identify genetic alterations distinguishing ITM from other melanoma metastases.

Main Methods:

  • Analyzed tumor samples from 528 patients using the MSK-IMPACT dataset (over 300 cancer-related genes).
  • Classified samples into primary, in-transit, regional lymph node, or distant metastases.
  • Compared driver mutation frequencies and used mutual information (MI) and principal component analysis (PCA) for pattern identification.

Main Results:

  • NRAS Q61 mutations were significantly enriched in ITM compared to other melanoma sites.
  • NF1 mutations were less frequent in ITM.
  • Identified NRAS-mutant/wild-type gene patterns potentially distinguishing ITM, with associated PI3K/AKT/mTOR, TGF-β, and E2F pathways.

Conclusions:

  • ITM exhibits a distinct molecular profile enriched for NRAS Q61 mutations.
  • ITM may have a lower co-mutation burden compared to other metastatic sites.
  • Findings provide a foundation for further research into ITM biology and clinical behavior.