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Related Experiment Video

Updated: Jul 16, 2026

An Enrichment Method for Small Extracellular Vesicles Derived from Liver Cancer Tissue
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An Enrichment Method for Small Extracellular Vesicles Derived from Liver Cancer Tissue

Published on: February 3, 2023

Circulating Extracellular Vesicles Reflect Dynamic Shifts in Liver Transcriptome Following Tumour Resection.

Lauren A Newman1, Daniel Daly2, Fiona Whelan3,4

  • 1Flinders Health and Medical Research Institute, College of Medicine and Public Health, Flinders University, Adelaide, SA 5042, Australia.

Cancers
|July 15, 2026
PubMed
Summary

Circulating extracellular vesicles (EVs) mirror liver cancer tumors at surgery but change post-resection. This dynamic shift shows EVs can monitor treatment response and recurrence risk.

Keywords:
blood biomarkersextracellular vesicleshepatocellular carcinomaliquid biopsymolecular oncology

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Last Updated: Jul 16, 2026

An Enrichment Method for Small Extracellular Vesicles Derived from Liver Cancer Tissue
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The Influence of Liver Resection on Intrahepatic Tumor Growth
07:55

The Influence of Liver Resection on Intrahepatic Tumor Growth

Published on: April 9, 2016

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Liver cancer diagnosis is often delayed, leading to poor outcomes.
  • High recurrence rates after surgery necessitate effective post-operative monitoring.
  • Non-invasive surveillance tools are crucial for managing liver cancer patients.

Purpose of the Study:

  • To assess circulating extracellular vesicles (EVs) as non-invasive biomarkers for liver cancer.
  • To investigate the dynamic molecular changes in EVs after tumor resection.
  • To determine if EVs can track patient response and recurrence post-surgery.

Main Methods:

  • Small ribonucleic acid (RNA) sequencing of matched tumor tissue, tissue-derived EVs, and plasma EVs.
  • Collection of plasma EVs at surgery and post-operative follow-up for longitudinal analysis.
  • Transcriptomic profiling to compare EV cargo with tumor molecular signatures.

Main Results:

  • Plasma EV transcriptomes closely matched tumor and tissue-derived EVs at surgery.
  • Post-operative plasma EVs showed significant divergence from the primary tumor profile.
  • A distinct shift in RNA cargo, including 173 new transcripts, was observed after tumor removal.

Conclusions:

  • Circulating EVs reflect the liver tumor's transcriptome at the time of resection.
  • EV profiles dynamically change after tumor removal, indicating clearance of tumor signals.
  • Longitudinal EV profiling offers a proof-of-concept for monitoring post-surgical changes and potential recurrence.