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Updated: Jul 16, 2026
08:50
A Nonsequencing Approach for the Rapid Detection of RNA Editing
Published on: April 21, 2022
Epitranscriptomic Analysis of A-to-I RNA Editing and m6A Using Short- and Long-Read Sequencing Technologies.
Nicholas Brenna1, Domenico Alessandro Silvestris2, Silvana Zugaro1
1Department of Onco-Haematology, Genetics and Epigenetics of Pediatric Tumours, Bambino Gesù Children's Hospital IRCCS, 00146 Rome, Italy.
International Journal of Molecular Sciences
|July 15, 2026
Summary
This review covers RNA modifications in human mRNA, focusing on methods to detect N6-methyladenosine (m6A) and adenosine-to-inosine (A-to-I) editing. It highlights current challenges and future research directions in this dynamic field.
Area of Science:
- Molecular Biology
- Genomics
- Biochemistry
Background:
- Over 160 post-transcriptional RNA modifications exist, showing diverse roles across human RNAs, cells, and tissues.
- Dynamic RNA modifications like N6-methyladenosine (m6A) and inosine (I) are increasingly detectable with high-throughput sequencing.
Purpose of the Study:
- To review RNA modifications in eukaryotic messenger RNA (mRNA).
- To provide an overview of high-throughput methodologies for detecting abundant adenosine-related modifications.
- To discuss current challenges and future research directions in the field of RNA modifications.
Main Methods:
- Focus on high-throughput sequencing technologies.
- Detailed overview of methodologies for detecting m6A.
- Detailed overview of methodologies for detecting adenosine-to-inosine (A-to-I) RNA editing.
Main Results:
- Identification of over 160 types of post-transcriptional RNA modifications.
- Systematic detection of dynamic RNA modifications, including m6A and inosine (I).
- Focus on adenosine-related modifications in eukaryotic mRNA.
Conclusions:
- RNA modifications are diverse and functionally significant.
- High-throughput sequencing enables systematic detection of dynamic RNA modifications.
- Further research is needed to address current challenges and explore future directions in RNA modification studies.
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