Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 16, 2026

Quantification of the Immunosuppressant Tacrolimus on Dried Blood Spots Using LC-MS/MS
08:38

Quantification of the Immunosuppressant Tacrolimus on Dried Blood Spots Using LC-MS/MS

Published on: November 8, 2015

A Novel Laboratory-Developed Test Using Multiplex qPCR to Further Personalize Tacrolimus Dosing.

Abhishek Chadha1, Zhiwei Wang1, Max Mamroth1

  • 1One Lambda Inc., Part of Thermo Fisher Scientific Inc.©, West Hills, CA 91304, USA.

International Journal of Molecular Sciences
|July 15, 2026
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Creating a hospital-based nutrition pathway for adults: evaluation of implementation in two United States hospital-systems.

Journal of eating disorders·2026
Same author

A Scoping Review of Health-Related Citizen Science Projects Involving Indigenous Peoples in Australia and Internationally.

Health promotion journal of Australia : official journal of Australian Association of Health Promotion Professionals·2025
Same author

Development of a novel qPCR test for monitoring immunosuppression levels by quantifying torque teno virus (TTV) in whole blood.

Human immunology·2025
Same author

Trailblazers in foregut surgery: Kyle Perry, MD (Ohio State University) interviews John Hunter, MD (Oregon Health and Science University).

Surgical endoscopy·2025
Same author

Integrating Australian Native Foods for a More Sustainable Food System: A Qualitative Co-Design Study with Aboriginal Communities.

International journal of environmental research and public health·2025
Same author

Spin wave Brillouin measurements in FeCo with a virtually imaged phased array spectrometer.

Optics express·2025

A new qPCR test, TacroType, accurately genotypes CYP3A5 variants. This aids in predicting tacrolimus metabolism, crucial for transplant patients, though routine genetic testing is not yet widespread.

Area of Science:

  • Pharmacogenomics
  • Clinical Chemistry
  • Molecular Diagnostics

Background:

  • Tacrolimus is a vital immunosuppressant in transplantation.
  • CYP3A5 gene polymorphisms significantly affect tacrolimus metabolism.
  • Routine pre-transplant CYP3A5 genotyping is not yet standard practice.

Purpose of the Study:

  • To evaluate the analytical performance of TacroType™, a novel qPCR-based assay for CYP3A5 genotyping.
  • To assess the accuracy and reproducibility of detecting common CYP3A5 variants (*3, *6, *7).

Main Methods:

  • Utilized a qPCR-based six-plex assay for CYP3A5 genotyping.
  • Conducted analytical performance studies following CLSI guidelines.
  • Validated accuracy using 32 reference samples and precision across operators, instruments, and reagent lots.
Keywords:
CYP3A5multiplexpharmacogeneticsqPCRtacrolimus

More Related Videos

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
07:27

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

Related Experiment Videos

Last Updated: Jul 16, 2026

Quantification of the Immunosuppressant Tacrolimus on Dried Blood Spots Using LC-MS/MS
08:38

Quantification of the Immunosuppressant Tacrolimus on Dried Blood Spots Using LC-MS/MS

Published on: November 8, 2015

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
07:27

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

Main Results:

  • TacroType demonstrated 100% genotyping accuracy across 4014 qPCR reactions.
  • Achieved a high report rate of 99.8% with consistent results from whole blood and buccal swabs.
  • Showed reliable performance across varying DNA concentrations, quality, operators, instruments, and reagent lots.

Conclusions:

  • TacroType provides accurate and reproducible CYP3A5 genotyping.
  • The assay is suitable for clinical use, potentially improving tacrolimus therapy management.
  • Facilitates personalized medicine in transplantation by identifying patients with altered drug metabolism.