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Related Experiment Video

Updated: Jul 16, 2026

Generation of Orthotopic Pancreatic Tumors and Ex vivo Characterization of Tumor-Infiltrating T Cell Cytotoxicity
06:16

Generation of Orthotopic Pancreatic Tumors and Ex vivo Characterization of Tumor-Infiltrating T Cell Cytotoxicity

Published on: December 7, 2019

Single-Cell Pan-Cancer Atlas Reveals GPR171 as a Candidate Marker of CD8+ T-Cell Dysfunction.

Xinyu Pan1, Ao Zhang1, Yuanyan Xiong1

  • 1Key Laboratory of Gene Engineering of the Ministry of Education, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.

International Journal of Molecular Sciences
|July 15, 2026
PubMed
Summary

Researchers identified GPR171 as a key marker of exhausted CD8+ T cells in various cancers. Reducing GPR171 activity may help restore antitumor immunity, offering a potential therapeutic target.

Keywords:
CD8+ T-cell exhaustionGPR171immune checkpointimmunosuppressiontumor microenvironment

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Area of Science:

  • Immunology
  • Oncology
  • Genomics

Background:

  • CD8+ T-cell exhaustion hinders cancer immunotherapy by promoting tumor immune evasion.
  • The molecular drivers of T-cell dysfunction across diverse cancers remain incompletely understood.

Purpose of the Study:

  • To identify molecular factors associated with exhausted CD8+ T cells in pan-cancer analysis.
  • To investigate the role of GPR171 in CD8+ T-cell dysfunction and its therapeutic potential.

Main Methods:

  • Integrated pan-cancer single-cell transcriptomic analysis.
  • Association analysis of GPR171 with gene expression profiles and immune regulatory genes.
  • Functional analysis of GPR171 activity in T cells.

Main Results:

  • GPR171 is commonly enriched in exhausted CD8+ T cells across multiple solid tumors.
  • GPR171 expression correlates with immunosuppressive and exhaustion-related gene programs, including CTLA4 and NR4A2.
  • Reduced GPR171 activity is linked to decreased exhaustion markers and enhanced cytotoxic/immune-activating programs, alongside a CREM-centered network.

Conclusions:

  • GPR171 serves as a potential pan-cancer marker for CD8+ T-cell dysfunction.
  • GPR171's association with immunosuppressive states highlights its potential as a therapeutic target to enhance antitumor immunity.