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A Descriptive Pilot Study of Endothelial Transcriptomic Responses to Extended Lactate Exposure In Vitro.

Daniel Conde^1,2, Gabriel Ibarra-Mejía³, Manuel Gomez^1,4

¹Clinical Applied Physiology (CAPh) Lab, The University of Texas at El Paso, El Paso, TX 79968, USA.

|July 15, 2026

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Lactate

Area of Science:

Endothelial Biology
Molecular Biology
Transcriptomics

Background:

Lactate is recognized as a signaling molecule influencing gene expression and vascular function.
Endothelial cells play a crucial role in vascular health and integrity.

Purpose of the Study:

To investigate the impact of varying lactate concentrations and exposure durations on the transcriptomic profile of human umbilical vein endothelial cells (HUVECs).
To identify specific genes modulated by lactate in endothelial cells.

Main Methods:

Descriptive pilot study utilizing next-generation RNA sequencing for unbiased, genome-wide transcriptomic analysis.
Focused analysis on genes related to endothelial biology, calcium signaling, and glycocalyx integrity.
Exposure of HUVECs to different lactate concentrations (0-30 mM) and time points (1-6 hours).

Keywords:

endothelium genes glycocalyx lactate next-generation sequencing

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Main Results:

No statistically significant effect of lactate concentration on the expression of examined genes was observed.
Prolonged incubation time correlated with differential expression of key genes including KLF2, KLF4, FOXO1, CD34, and VCAM1.
Transcriptomic changes were primarily associated with incubation duration rather than lactate levels under static conditions.

Conclusions:

Incubation time, not lactate concentration, appears to be a significant factor in modulating endothelial gene expression under static experimental conditions.
Preliminary transcriptomic data suggest potential roles for incubation duration in endothelial cell responses.
Further mechanistic studies, including functional and protein-level analyses, are warranted to elucidate lactate's role in endothelial biology.