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Updated: Jul 16, 2026

Soybean Hairy Root Transformation for the Analysis of Gene Function
Published on: May 5, 2023
Genome-Wide Identification, Expression and Tissue-Specific Epigenetic Modification Analysis of the Su(var)3-9 SET
Min Wang1, Wei Zhou2, Zihui Zhang3
1College of Agriculture and Forestry Science and Technology, Hebei North University, Zhangjiakou 075000, China.
Background:
Su(var)3-9 SET genes encode key histone methyltransferases that catalyze H3K9 methylation, a modification generally associated with heterochromatin formation and transcriptional repression.
Methods:
We identified 23 GmSu(var)3-9 SETs and systematically characterized their tissue-specific epigenetic modifications as well as their phylogenetic relationships, chromosomal distributions, conserved domains, gene structures, GO annotations, collinearity, cis-regulatory elements, and expression profiles across diverse tissues and under salt stress.
Results:
These genes were divided into seven groups, exhibiting diverse structures and uneven distribution on chromosomes. Gene structure and conserved motif analyses revealed high structural diversity among family members, with variations in intron-exon distribution, conserved motifs, and functional domains. Promoter analysis detected multiple cis elements responsive to light, hormones, and abiotic stresses. Most genes showed preferential expression in meristems, roots, and leaves, and responded to salt stress. Co-expression network analysis revealed that these genes were co-expressed with stress- and development-related genes. GmSUVH12 histone modifications exhibited obvious tissue specificity.
Conclusions:
Overall, these results provided insights into the evolutionary and functional roles of GmSu(var)3-9 SETs in soybean.
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