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Related Experiment Video

Updated: Jul 16, 2026

Modelling Zika Virus Infection of the Developing Human Brain In Vitro Using Stem Cell Derived Cerebral Organoids
09:18

Modelling Zika Virus Infection of the Developing Human Brain In Vitro Using Stem Cell Derived Cerebral Organoids

Published on: September 19, 2017

Chiropteran (Hypsugo savii) Post-Natal Brain 2D-In Vitro Models: Primary Cell Isolation, Immortalization and

Antonella Molinari1, Valentina Moccia1, Massimiliano Babbucci1

  • 1Department of Comparative Biomedicine and Food Science, University of Padua, 35020 Legnaro, Italy.

Animals : an Open Access Journal From MDPI
|July 15, 2026
PubMed
Summary

Researchers developed new bat brain cell models for studying zoonotic diseases. These 2D *in vitro* models from *Hypsugo savii* bats aid research into viral tolerance and neuroinflammation.

Keywords:
2D-in vitro modelsOne Healthbatbrainelectroporationimmortalized cellsimmunityliposome-mediated transfectionprimary cells

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Published on: September 15, 2014

Area of Science:

  • Neuroscience
  • Virology
  • Cell Biology

Background:

  • Bats are key reservoirs for zoonotic pathogens.
  • Existing chiropteran neural *in vitro* models are limited.
  • Understanding bat neurobiology is crucial for One Health initiatives.

Purpose of the Study:

  • Establish and characterize novel chiropteran brain-derived 2D *in vitro* cell models.
  • Develop immortalized cell lines for extended research.
  • Provide a platform for studying bat neurobiology and infectious diseases.

Main Methods:

  • Primary chiropteran brain cells (CpBCs) were isolated from *Hypsugo savii*.
  • Immortalization was attempted using Simian virus 40 large T antigen (SV40) and human telomerase reverse transcriptase (hTERT).
  • SV40-mediated electroporation successfully generated immortalized chiropteran brain cells (CiBCs).
  • RNA sequencing was performed to compare transcriptional profiles of CpBCs and CiBCs.

Main Results:

  • SV40 transfection yielded immortalized CiBCs; hTERT and liposome methods failed.
  • CiBCs showed upregulated cell cycle and proliferation pathways compared to CpBCs.
  • CiBCs displayed downregulated apoptosis, inflammation, and immune pathways.
  • SV40 immortalization induced partial dedifferentiation, with neural stem cell-like and cancer-associated signatures.

Conclusions:

  • The study presents the first 2D *in vitro* platform using immortalized bat brain cells.
  • This novel platform facilitates research into bat neurobiology and host-pathogen interactions.
  • The models are valuable for studying viral tolerance and neurotropic diseases in bats, relevant to zoonoses.