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Staphylococcus aureus H autolytic activity: general properties.

I Takebe, H J Singer, E M Wise

    Journal of Bacteriology
    |April 1, 1970
    PubMed
    Summary
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    Staphylococcus aureus strain H exhibits autolytic activity, primarily in its cell wall. This activity significantly increases when acting on murein from penicillin-treated cells compared to normal cells.

    Area of Science:

    • Microbiology
    • Biochemistry

    Background:

    • Staphylococcus aureus is a significant human pathogen.
    • Bacterial cell wall autolysis plays a role in cell wall remodeling and antibiotic resistance.

    Purpose of the Study:

    • To characterize the autolytic activity of Staphylococcus aureus strain H.
    • To investigate the substrate specificity of this autolytic activity.

    Main Methods:

    • Isolation of autolytic enzymes from high-speed supernatant fractions of broken-cell preparations.
    • Measurement of enzyme activity using turbidimetric and radioactive assays.
    • Comparison of activity on murein from normal versus penicillin-treated cells.

    Main Results:

    • Autolytic activity was detected in cell wall fractions but was most concentrated in supernatant fractions.

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  • The autolytic activity was significantly higher on murein extracted from penicillin-treated cells compared to murein from normal cells.

    • Conclusions:

    • Staphylococcus aureus strain H possesses cell wall autolytic activity.
    • This activity is enhanced when targeting murein from cells exposed to penicillin, suggesting a potential mechanism for antibiotic-induced cell wall degradation.