Effects of Water-Soluble C₆₀ Fullerenes on Rat Musculus Soleus Contraction Following Neurogenic Atrophy

Area of Science:

• Biomedical Engineering
• Neuroscience
• Biochemistry

Background:

• Neurogenic atrophy, a severe muscle wasting condition, results from nerve damage.
• Sciatic nerve injury triggers molecular changes leading to muscle dysfunction and atrophy.
• Oxidative stress is a key factor in the development of neurogenic atrophy.

Purpose of the Study:

• To investigate the therapeutic effects of water-soluble C60 fullerenes on neurogenic atrophy.
• To evaluate the impact of C60 fullerenes on muscle contraction dynamics and biochemical markers post-sciatic nerve injury.
• To assess the time-dependent efficacy of C60 fullerenes in a rat model.

Main Methods:

• Oral administration of water-soluble C60 fullerenes (1 mg/kg/day) to rats with induced sciatic nerve transection.
• Assessment of biomechanical parameters (contraction forces, power) using tensometry.
• Analysis of blood biochemical markers (CRP, lactate, glutathione, SOD, catalase) and histological muscle damage indicators.

Main Results:

• Water-soluble C60 fullerenes significantly improved musculus soleus contractile activity in a time-dependent manner.
• By day 45, biomechanical parameters showed a 23-35% improvement, with biochemical markers nearing control levels.
• Histological analysis revealed reduced muscle fiber destruction and fibrosis.

Conclusions:

• Water-soluble C60 fullerenes demonstrate significant therapeutic potential for neurogenic atrophy.
• The antioxidant properties of C60 fullerenes mitigate muscle damage and dysfunction following peripheral nerve injury.
• This study supports the application of C60 fullerenes in treating muscle pathologies linked to nerve damage.