Fully Automated Azeotropic Drying-Free Synthesis of [¹⁸F]SynVesT-1 via Copper-Mediated Fluorination Using the Trasis...

Area of Science:

• Neuroscience
• Radiochemistry
• Medical Imaging

Background:

• Synaptic vesicle glycoprotein 2A (SV2A) is a key presynaptic protein.
• SV2A PET imaging offers in vivo assessment of synaptic density for neurological disease research.
• Previous [18F]SynVesT-1 synthesis relied on a copper-mediated fluorination requiring azeotropic drying.

Purpose of the Study:

• To develop a fully automated and reproducible synthesis of the SV2A PET tracer [18F]SynVesT-1.
• To optimize the radiolabeling strategy using an anion-exchange cartridge elution method.

Main Methods:

• Utilized a previously established radiolabeling strategy eluting [18F]fluoride as DMAPH·[18F]F.
• Employed the Trasis All-in-One (AIO) module for automated synthesis.
• Performed copper-mediated fluorination without azeotropic drying.

Main Results:

• Achieved a total synthesis time of 60 minutes.
• Obtained superior overall decay-corrected radiochemical yields (30-37%) compared to previous methods (20.6 ± 1.2%).
• Required reduced precursor mass (2 mg vs. 5 mg) with >98% radiochemical purity.

Conclusions:

• The automated Trasis AIO module method provides a reproducible and efficient synthesis of [18F]SynVesT-1.
• This optimized method enhances radiochemical yield and reduces precursor requirements for SV2A PET tracer production.
• Facilitates broader application of SV2A PET imaging in neuropsychiatric and neurodegenerative disease studies.