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Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration
Published on: September 12, 2014
Gentamicin-Loaded Electrospun PVA/Kefiran/Schizophyllan Membrane for Skin Tissue Engineering Applications
Karla Katiushka Solís-Arévalo1, Luis J Galán-Wong1, Aida Rodriguez-Garcia1
1Universidad Autónoma de Nuevo León, Facultad de Ciencias Biológicas, Instituto de Biotecnología, Ciudad Universitaria, Av. Pedro de Alba S/N, San Nicolás de los Garza C.P. 66455, Nuevo León, Mexico.
Polymers
|July 15, 2026
Summary
New electrospun membranes offer a promising solution for wound care, reducing skin damage and infection risk. These biocompatible materials show significant antimicrobial activity and are safe for skin cells.
Area of Science:
- Biomaterials Science
- Infectious Disease Research
- Regenerative Medicine
Background:
- Healthcare-associated infections are a significant concern in clinical settings.
- Traditional wound dressings can damage the skin barrier upon removal.
- Novel biomaterials are needed for advanced wound management.
Purpose of the Study:
- To develop and characterize an electrospun membrane for wound dressing applications.
- To incorporate gentamicin and ascorbic acid for enhanced therapeutic effects.
- To evaluate the antimicrobial and cytocompatibility properties of the novel membrane.
Main Methods:
- Electrospinning of polyvinyl alcohol, kefiran, and schizophyllan.
- Loading the membrane with gentamicin and ascorbic acid.
- Assessing antimicrobial activity against *Staphylococcus aureus* and *Pseudomonas aeruginosa*.
- Evaluating cytocompatibility using human dermal fibroblasts (HDFn cells).
Main Results:
- Beadless electrospun membranes with a 400 nm diameter were successfully produced.
- Significant antimicrobial activity was observed against *Pseudomonas aeruginosa* (16.4 ± 2.2 mm inhibition halos).
- The membrane demonstrated no cytotoxicity to human dermal fibroblasts, indicating good biocompatibility.
Conclusions:
- The developed electrospun membrane exhibits potent antimicrobial properties and excellent cytocompatibility.
- This novel biomaterial holds significant potential for future wound dressing applications.
- The study highlights a promising advancement in managing skin wounds and preventing infections.
