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Adaptability of Cytoskeletal Filaments

Adaptability of Cytoskeletal Filaments

The cytoskeleton is a complex dynamic structure performing varied functions based on cellular requirements. The adaptability of the individual filaments in the cytoskeleton determines their ability to perform various functions within the cell. It can undergo rapid reorganization during processes like cell division or remain stable for several hours as in the interphase. The adaptability of these filaments depends on stringent regulatory mechanisms. The microfilament and microtubules of the...

Actin Filament Depolymerization

Actin Filament Depolymerization

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Arp2/3 Complex
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Related Experiment Video

Updated: Jul 16, 2026

Aip1p Dynamics Are Altered by the R256H Mutation in Actin

Aip1p Dynamics Are Altered by the R256H Mutation in Actin

Published on: July 30, 2014

The OsMYB30-OsADF7 Axis Modulates Rice Heat Acclimation Through Actin Microfilament Dynamics.

Tianying Ren¹, Pan Li¹, Zhuoqun Liu¹

¹State Key Laboratory of Macromolecular Drugs and Large-Scale Preparation, Shandong Key Laboratory of Applied Technology for Protein and Peptide Drugs, School of Pharmaceutical Sciences and Food Engineering, Liaocheng University, Liaocheng 252059, China.

Plants (Basel, Switzerland)

|July 15, 2026

Summary

Rice heat acclimation involves the actin cytoskeleton. OsMYB30 regulates OsADF7, impacting heat tolerance by modulating microfilament dynamics for improved rice thermotolerance.

Keywords:

Oryza sativa actin depolymerization factor heat tolerance transcription factor

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Area of Science:

Plant molecular biology
Cellular stress response
Plant genetics

Background:

The actin cytoskeleton is crucial for plant stress signaling and homeostasis.
While plant heat stress adaptation is known, rice thermotolerance regulatory networks are unclear.

Purpose of the Study:

Investigate the roles of OsADF7 and OsMYB30 in rice heat acclimation.
Elucidate the transcriptional and cytoskeletal mechanisms governing rice thermotolerance.

Main Methods:

Gene knockout and overexpression of OsADF7 and OsMYB30 in rice.
Analysis of microfilament dynamics and physiological heat stress responses.
Promoter analysis and transcription factor binding assays.

Main Results:

OsADF7 negatively regulates heat acclimation; its knockout enhances tolerance.
OsMYB30 transcriptionally controls OsADF7, with both genes suppressed by heat stress.
OsMYB30 knockout improves heat tolerance, while its overexpression increases OsADF7 and heat sensitivity.

Conclusions:

The OsMYB30-OsADF7 module regulates actin cytoskeleton dynamics in rice heat acclimation.
This pathway provides mechanistic insights into plant thermotolerance.
OsADF7 and OsMYB30 are potential targets for improving heat-resistant rice varieties.