Related Experiment Video
Updated: Jul 16, 2026

Plant-Microbe Interaction: Transcriptional Response of Bacillus Mycoides to Potato Root Exudates
Published on: July 2, 2018
Distinct functional responses of root endophyte and rhizosphere microbial communities in intercropping systems under
Sulamita Santos Correa1, Aysha Hamad Mohammed Asayan Almansoori1, Shahnaz Nazzar1,2
1Department of Biology, College of Science, United Arab Emirates University, Al Ain, United Arab Emirates.
Introduction:
Sustainable strategies have been implemented to enhance plant development and productivity, including intercropping systems. This approach is particularly effective in arid regions, where diverse microbial populations associated with intercropping plants contribute significantly to stress tolerance and plant growthpromoting.
Methods:
We evaluated how intercropping and monocropping systems influence the diversity, functional traits, and stress tolerance of root-associated bacteria. Root endophytic and rhizosphere bacteria were isolated from intercropping and monocropping systems under arid conditions and evaluated for plant growth-promoting traits, enzymatic activities, exopolysaccharide and cellulose production, biofilm formation under drought stress, and tolerance to drought, salinity, and heat stress.
Results And Discussion:
Eighty bacterial isolates were characterized, most of which exhibited multiple plant growth-promoting traits and tolerance to environmental stress. Overall, Bacillus spp. were the dominant bacteria among endophyte and rhizosphere communities. In alfalfa-broad and Egyptian wheat-broad intercropping, Bacillus spp. were more common, whereas Pseudomonas spp. were more common in barley-mustard intercropping. Multivariate analysis of functional traits (NMDS) revealed that bacterial communities were primarily structured by niche (endophytic vs. rhizosphere) rather than by intercropping type. The results suggest that rhizosphere bacteria associated with intercropping enhanced nitrogen fixation compared to those from monocropping systems, and the exopolysaccharide produced by endophytic isolates from intercropping using glucose as a carbon source varied from monocropping. Based on our results, the intercropping system creates a favorable microenvironment for certain bacteria, such as Bacillus spp. and Pseudomonas spp., which possess specific plant growth-promoting traits suitable for harsh environments, such as arid regions. These findings support other studies showing that bacteria adapted to extreme conditions, and isolated from diverse and multiple cropping systems, can function as plant bioinoculants, supporting plant species under adverse conditions.
Related Concept Videos
Microbe-Plant Interactions
Microbial Interactions: Mutualism
Responses to Drought and Flooding
Epiphytes, Parasites, and Carnivores
The Roles of Bacteria and Fungi in Plant Nutrition
Soil Microbial Ecology

