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Related Experiment Video

Updated: Jul 16, 2026

Isolated Lung Perfusion System in the Rabbit Model
10:18

Isolated Lung Perfusion System in the Rabbit Model

Published on: July 15, 2021

Intestinal preservation using a tailored luminal solution and aeration: A small animal study.

Jasmine Bagge1,2,3, Arvind Manikantan Padma4, Anna Casselbrant3

  • 1The Transplant Institute, Sahlgrenska University Hospital, Gothenburg, Sweden.

Intestinal Failure (New York, N.Y.)
|July 15, 2026
PubMed
Summary

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Haematologica·2026

Preserving intestinal grafts with a luminal solution of polyethylene glycol, glutamine, and arginine (PEG-GAs) reduced tissue injury compared to static cold storage (SCS). Air insufflation offered no additional benefit for intestinal preservation.

Area of Science:

  • Transplantation research
  • Organ preservation techniques
  • Gastrointestinal surgery

Background:

  • Oxygenation during organ preservation may improve transplant outcomes, but data for intestinal preservation is limited.
  • Static cold storage (SCS) is a standard method for organ preservation.
  • Novel preservation solutions aim to mitigate ischemia-reperfusion injury.

Purpose of the Study:

  • To compare the efficacy of static cold storage (SCS) with novel preservation strategies for rat intestinal grafts.
  • To evaluate the impact of a luminal polyethylene glycol solution (PEG-GAs) with or without air insufflation on intestinal preservation.
  • To assess histological and molecular changes in intestinal tissue after varying cold storage durations.

Main Methods:

  • Rat small intestines were preserved using SCS, luminal PEG-GAs, or luminal PEG-GAs with air insufflation.
Keywords:
Amino acidsInsufflationIntestinalIschemia-reperfusionTight junctionTransplantation

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Last Updated: Jul 16, 2026

Isolated Lung Perfusion System in the Rabbit Model
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Published on: July 15, 2021

Establishment of a Novel Ex Vivo Lung Perfusion System for Rat Lungs After Circulatory Death
09:14

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Published on: October 18, 2024

  • Tissue samples were collected after 8, 14, and 24 hours of cold storage.
  • Histological assessment and Western blot analysis were performed to evaluate tissue injury and protein expression.

    • Main Results:

    • Intestinal grafts preserved with PEG-GAs (with or without air) showed reduced histological injury scores compared to SCS.
    • Goblet cell depletion and tight junction protein (zonula occludens-1, claudin, occludin) disruption were less pronounced in PEG-GAs groups.
    • Elevated caspase 3 expression, indicative of apoptosis, was observed in the SCS group.

    Conclusions:

    • Static cold storage leads to significant tissue and molecular damage in intestinal grafts within 8 hours.
    • Luminal preservation with PEG-GAs offers a protective effect against intestinal injury during cold storage.
    • Additional air insufflation did not provide significant benefits over luminal preservation alone.