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Related Concept Videos

In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

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ADAM20 Participates Modestly to Fertilization as Its Absence Leads to In Vitro Hypofertility in Mouse.

Marie-Sophie Girault1, Sophie Dupuis1, Rémi Pierre1

  • 1Institut Cochin, INSERM, CNRS, Université Paris Cité, Paris, France.

Cell Biochemistry and Function
|July 15, 2026
PubMed
Summary

The ADAM20 gene is not essential for mouse fertility, as knock-out males remain fertile in vivo. However, ADAM20, ADAM25, and ADAM39 genes modestly contribute to the fertilization process.

Keywords:
fertilityfertilizationinfertilityknock‐outmalemousespermtestis

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Determination of Reproductive Competence by Confirming Pubertal Onset and Performing a Fertility Assay in Mice and Rats
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Published on: October 13, 2018

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Last Updated: Jul 16, 2026

Mouse Round Spermatid Injection
08:41

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Determination of Reproductive Competence by Confirming Pubertal Onset and Performing a Fertility Assay in Mice and Rats
06:38

Determination of Reproductive Competence by Confirming Pubertal Onset and Performing a Fertility Assay in Mice and Rats

Published on: October 13, 2018

Area of Science:

  • Reproductive Biology
  • Genetics
  • Molecular Biology

Background:

  • A mutation in the ADAM20 gene has been linked to male infertility.
  • ADAM20's precise role in mammalian fertilization remains unclear.
  • ADAM20, ADAM25, and ADAM39 are homologous genes with potential overlapping functions.

Purpose of the Study:

  • To investigate the function of ADAM20 in male fertility using a mouse model.
  • To determine if ADAM25 and ADAM39 compensate for the loss of ADAM20.
  • To elucidate the contribution of ADAM20, ADAM25, and ADAM39 to the fertilization process.

Main Methods:

  • Generation of Adam20 knock-out (KO) mice.
  • Assessment of in vivo fertility, sperm count, and morphology in Adam20-KO males.
  • Performance of in vitro fertilization (IVF) assays.
  • Generation of a triple KO mouse model lacking Adam20, Adam25, and Adam39.

Main Results:

  • Adam20-KO males exhibited normal in vivo fertility and sperm parameters.
  • Adam20-KO males showed reduced fertility in in vitro fertilization assays.
  • Triple KO males displayed a phenotype identical to single Adam20-KO males, indicating no compensatory effect.

Conclusions:

  • ADAM20, ADAM25, and ADAM39 are dispensable for normal male fertility in mice.
  • These genes play a modest, non-essential role in the fertilization process.
  • ADAM20 deficiency primarily impacts in vitro fertilization efficiency.