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Related Concept Videos

Epigenetic Regulation01:37

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
Epigenetic Regulation01:46

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: Jul 16, 2026

Determination of DNA Methylation of Imprinted Genes in Arabidopsis Endosperm
09:23

Determination of DNA Methylation of Imprinted Genes in Arabidopsis Endosperm

Published on: January 28, 2011

Engineering DNA Methylation Dynamics in Plants: Strategies from Detection to Deployment.

Anita Choudhary1,2, Vijay Gahlaut3, Vandana Jaiswal1,2

  • 1Biotechnology, Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, 176061, India.

Journal of Experimental Botany
|July 15, 2026
PubMed
Summary

Site-specific DNA methylation editing in plants allows for targeted manipulation of gene expression and traits. This epigenetic approach offers a powerful new tool for crop improvement without altering the DNA sequence.

Keywords:
DNA methylationbreedingepigenetic modificationphenotypic variationtraitwhole genome bisulfite sequencing

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Last Updated: Jul 16, 2026

Determination of DNA Methylation of Imprinted Genes in Arabidopsis Endosperm
09:23

Determination of DNA Methylation of Imprinted Genes in Arabidopsis Endosperm

Published on: January 28, 2011

Detection of Histone Modifications in Plant Leaves
07:08

Detection of Histone Modifications in Plant Leaves

Published on: September 23, 2011

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
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Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Published on: September 7, 2017

Area of Science:

  • Plant epigenetics
  • Molecular biology
  • Agricultural science

Background:

  • DNA methylation is a key epigenetic mark regulating gene expression, genomic stability, and development in plants.
  • Advances in sequencing identify many DNA methylation sites, but targeted manipulation is crucial for crop improvement.

Purpose of the Study:

  • To review recent breakthroughs in site-specific DNA methylation and demethylation in plants.
  • To evaluate methods for detecting DNA methylation and analyze DNA-binding protein recruitment.
  • To provide guidance on effective strategies for DNA methylation editing in crop breeding.

Main Methods:

  • Review of current literature on site-specific DNA methylation and demethylation techniques.
  • Evaluation of methods for DNA methylation detection in crop plants.
  • Examination of DNA-binding protein recruitment to target loci.

Main Results:

  • Recent advances enable precise investigation and manipulation of DNA methylation at specific loci in plants.
  • Various methods for DNA methylation detection have distinct advantages and limitations.
  • Understanding DNA-binding protein recruitment aids in targeting epigenetic modifications.

Conclusions:

  • Site-specific DNA methylation editing offers a transformative approach for crop improvement.
  • This technology can unlock phenotypic variation without changing the underlying DNA sequence.
  • Epigenetic editing holds significant potential for accelerating agricultural advancements.