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Related Experiment Video

Updated: Jul 16, 2026

High-resolution Imaging of Nuclear Dynamics in Live Cells under Uniaxial Tensile Strain

High-resolution Imaging of Nuclear Dynamics in Live Cells under Uniaxial Tensile Strain

Published on: June 2, 2019

Nuclear Dynamics and Its Timing Regulation Revealed by Live-Cell Imaging.

Haruka Oda¹, Nao Yonezawa¹, Kazuo Yamagata²

¹Faculty of Biology-Oriented Science and Technology, Kindai University, Wakayama, Japan.

Advances in Experimental Medicine and Biology

|July 15, 2026

Summary

Early development involves rapid genome replication and epigenetic changes. Live-cell imaging helps quantitatively analyze these spatiotemporal nuclear events across species.

Keywords:

DNA methylation Developmental timer Histone modification Live-cell imaging Mouse zygotic genome activation Nuclear dynamics

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Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations

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Published on: September 20, 2019

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Last Updated: Jul 16, 2026

High-resolution Imaging of Nuclear Dynamics in Live Cells under Uniaxial Tensile Strain

High-resolution Imaging of Nuclear Dynamics in Live Cells under Uniaxial Tensile Strain

Published on: June 2, 2019

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations

Published on: September 20, 2019

Area of Science:

Developmental Biology
Epigenetics
Cell Biology

Background:

Biological phenomena are critically controlled by time, especially during early development.
The transition from gametes to zygotes involves rapid genome replication and epigenetic reprogramming.
Nuclear events like genome replication and chromosome segregation occur rapidly during this period.

Purpose of the Study:

To review research on spatiotemporal changes within and outside cell nuclei during early development.
To highlight live-cell imaging as a key technology for quantitative analysis of these dynamic processes.

Main Methods:

Review of historical research on nuclear and cellular changes.
Application of single-cell omics analyses.
Utilization of high-throughput chromosome conformation capture (Hi-C) data.
Employing live-cell imaging technologies for quantitative analysis.

Main Results:

Dynamic remodeling of epigenetic information, including histone modifications and DNA methylation.
Strict control over genome, chromatin architecture, and nuclear positioning of gene loci.
Single-cell omics and Hi-C data provide locus-level resolution of genomic structures.
Spatiotemporal changes occur across species, with variations in developmental timing.

Conclusions:

Understanding the precise timing of nuclear events is crucial for early development.
Live-cell imaging offers powerful quantitative insights into dynamic cellular processes.
Interspecies variations in developmental speed influence the timing of these genomic events.