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Updated: Jul 16, 2026
09:20
High-resolution Imaging of Nuclear Dynamics in Live Cells under Uniaxial Tensile Strain
Published on: June 2, 2019
Nuclear Dynamics and Its Timing Regulation Revealed by Live-Cell Imaging
Haruka Oda1, Nao Yonezawa1, Kazuo Yamagata2
1Faculty of Biology-Oriented Science and Technology, Kindai University, Wakayama, Japan.
Advances in Experimental Medicine and Biology
|July 15, 2026
Summary
Early development involves rapid genome replication and epigenetic changes. Live-cell imaging helps quantitatively analyze these spatiotemporal nuclear events across species.
Area of Science:
- Developmental Biology
- Epigenetics
- Cell Biology
Background:
- Biological phenomena are critically controlled by time, especially during early development.
- The transition from gametes to zygotes involves rapid genome replication and epigenetic reprogramming.
- Nuclear events like genome replication and chromosome segregation occur rapidly during this period.
Purpose of the Study:
- To review research on spatiotemporal changes within and outside cell nuclei during early development.
- To highlight live-cell imaging as a key technology for quantitative analysis of these dynamic processes.
Main Methods:
- Review of historical research on nuclear and cellular changes.
- Application of single-cell omics analyses.
- Utilization of high-throughput chromosome conformation capture (Hi-C) data.
- Employing live-cell imaging technologies for quantitative analysis.
Main Results:
- Dynamic remodeling of epigenetic information, including histone modifications and DNA methylation.
- Strict control over genome, chromatin architecture, and nuclear positioning of gene loci.
- Single-cell omics and Hi-C data provide locus-level resolution of genomic structures.
- Spatiotemporal changes occur across species, with variations in developmental timing.
Conclusions:
- Understanding the precise timing of nuclear events is crucial for early development.
- Live-cell imaging offers powerful quantitative insights into dynamic cellular processes.
- Interspecies variations in developmental speed influence the timing of these genomic events.
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