Mitogenome and Phylogeny of the Hairy Puffer Pao baileyi (Sontirat 1985)
Wei Hu1, Xiao-Die Chen1, Xiao Ma1
1College of Life Sciences, Nanjing Forestry University, Nanjing, 210037, China.
Abstract:
The mitogenomics of Pao baileyi (Sontirat 1985), a tropical pufferfish with ornamental and research value, remain unreported. To understand its molecular characteristics and enrich mitogenome data for Tetraodontidae fish, in this study, we focused on the mitogenome of wild P. baileyi. The complete mitogenome sequence was obtained through high-throughput sequencing, and systematic analysis of the structural characteristics of the genome, base composition, and codon usage bias was conducted. A phylogenetic tree was constructed using Monacanthidae species as outgroups to explore the position of P. baileyi within Tetraodontidae. Results showed that the P. baileyi mitogenome is a closed-loop double-stranded DNA molecule with gene composition and arrangement conforming to Tetraodontidae mitogenome characteristics. It contains 13 protein-coding genes, 22 tRNAs, 2 rRNAs, and 1 control region. The base composition has an AT bias, codon usage exhibits specific preferences, and all tRNA genes have a cloverleaf secondary structure. Phylogenetic analysis revealed that P. baileyi is closely related to other Pao species and forms a distinct evolutionary branch with other genera and species in Tetraodontidae, providing a basis for species classification. This is the first report of the complete mitogenome characteristics of P. baileyi, providing basic data for phylogenetic relationships, germplasm resource conservation, and ornamental breeding research on Tetraodontidae fish.
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