Protocol for simultaneous non-invasive electrocardiogram and respiratory plethysmography recordings in mice

Here, we present a protocol for non-invasive simultaneous recording of electrocardiogram (ECG) and breathing in conscious mice. We describe steps for securing surface ECG electrodes with kinesiology tape, lightly sedating mice for lead placement, and monitoring via plethysmography. Heart (electrocardiogram) and lung signals (air flow) were synchronously acquired, enabling accurate, minimally invasive cardiopulmonary phenotyping for neuroscience research and predictive therapeutic interventions (pharmacological, genetic, and engineering), without surgical implantation or confounding anesthesia.