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Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation
Published on: September 19, 2017
Electrochemical Biosensor on a Microfluidic Coculture Chip for Ovalbumin Detection
Donglei Jiang1, Luwen Ye1, Zeng Feng1
1College of Food Science and Engineering, Collaborative Innovation Center for Modern Grain Circulation and Safety, Key Laboratory of Grains and Oils Quality Control and Processing, Nanjing University of Finance and Economics, Nanjing210023, Jiangsu, P.R. China.
Abstract:
In this study, a microfluidic coculture electrochemical sensing platform was developed for the sensitive detection of ovalbumin (OVA). The chip integrated dual-layer serpentine microchannels separated by a porous PET membrane, enabling coculture and communication between Caco-2 and RBL-2H3 cells. OVA was quantified indirectly by monitoring interleukin-6 (IL-6) released during the cellular allergic response. The screen-printed electrode was modified by diazonium grafting, followed by AuNP electrodeposition for antibody immobilization and signal amplification. The integrated platform exhibited a linear response to OVA from 0.2 to 4 pg/mL, with a calibration equation of Ret(kΩ) = -7.450COVA(kΩ·mL/pg) + 47.342 (R2 = 0.998) and a detection limit of 0.161 pg/mL. A maximum electrochemical response was observed after 4 h of OVA stimulation, allowing dynamic monitoring of allergen-induced cellular responses. The proposed platform provides a sensitive and biomimetic strategy for response-oriented food allergen analysis.
