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Updated: Jul 17, 2026

Live Imaging Followed by Single Cell Tracking to Monitor Cell Biology and the Lineage Progression of Multiple Neural Populations

Live Imaging Followed by Single Cell Tracking to Monitor Cell Biology and the Lineage Progression of Multiple Neural Populations

Published on: December 16, 2017

A pipeline for cell migration analysis in live-cell imaging data from human iPSC-derived forebrain assembloids.

Maya P Weidman¹, Natalie Baker Campbell², Cody Headings¹

¹Department of Biomedical Engineering, Boston University, Boston, MA, United States.

Frontiers in Cell and Developmental Biology

|July 16, 2026

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Human induced pluripotent stem cell-derived forebrain assembloids (FAs) enable in vitro study of cell migration. A new processing pipeline tracks cell movement, revealing super-diffusive directed motility in developing forebrain cells.

Area of Science:

Neuroscience
Developmental Biology
Stem Cell Biology

Background:

Inhibitory interneurons and oligodendrocyte progenitor cells migrate extensively during forebrain development.
Human induced pluripotent stem cell-derived forebrain assembloids (FAs) offer an in vitro model for studying cell migration.

Purpose of the Study:

To develop and validate a processing pipeline for tracking cell migration in human iPSC-derived forebrain assembloids.
To analyze the migratory behavior of key cell types within these 3D models.

Main Methods:

Utilized viral labeling (EYFP and tdTomato) to track cells in FAs.
Employed spinning disk confocal microscopy for 4D imaging over 15-17 hours.
Developed an end-to-end pipeline including preprocessing, manual tracking, and trajectory analysis.

Keywords:

4D image analysis forebrain assembloids interneuron migration live-cell imaging manual cell tracking oligodendrocyte migration

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An Organotypic Slice Assay for High-Resolution Time-Lapse Imaging of Neuronal Migration in the Postnatal Brain

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Published on: December 11, 2010

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Last Updated: Jul 17, 2026

Live Imaging Followed by Single Cell Tracking to Monitor Cell Biology and the Lineage Progression of Multiple Neural Populations

Live Imaging Followed by Single Cell Tracking to Monitor Cell Biology and the Lineage Progression of Multiple Neural Populations

Published on: December 16, 2017

An Organotypic Slice Assay for High-Resolution Time-Lapse Imaging of Neuronal Migration in the Postnatal Brain

An Organotypic Slice Assay for High-Resolution Time-Lapse Imaging of Neuronal Migration in the Postnatal Brain

Published on: December 11, 2010

Main Results:

Image preprocessing enhanced data quality for tracking.
Analysis of 673 cells revealed super-diffusive directed motility.
The developed pipeline is a valuable resource for cell tracking in FAs.

Conclusions:

The new pipeline facilitates robust cell tracking in iPSC-derived FAs.
This resource aids in understanding cellular migration dynamics in developing brain models.
The pipeline can serve as a benchmark for developing automated tracking algorithms.