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Updated: Jul 17, 2026
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Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)
Published on: August 4, 2018
Multi-orientation non-confocal retinal imaging in an AOSLO using a Dove prism.
Ruth E Woehlke1, Robert F Cooper1,2
1Joint Department of Biomedical Engineering, Marquette University and Medical College of Wisconsin, Milwaukee WI, 53226, USA.
Biomedical Optics Express
|July 16, 2026
Summary
Adaptive optics scanning light ophthalmoscopes with a Dove prism improve imaging of retinal structures. This technique enhances contrast and sensitivity for visualizing the retina's topography.
Area of Science:
- Ophthalmology
- Optical Imaging
- Biomedical Engineering
Background:
- Adaptive optics scanning light ophthalmoscopes (AOSLO) offer high-resolution retinal imaging.
- Two-detector split-detection arrangements in AOSLO produce phase contrast images of retinal topography.
- Standard systems using edge mirrors have limitations in resolving structures perpendicular to the split mirror axis.
Purpose of the Study:
- To introduce and evaluate a novel method for improving split-detection imaging in AOSLO.
- To overcome the contrast limitations of standard two-detector systems for retinal structure visualization.
Main Methods:
- Implemented a Dove prism to rotate the light path within a two-detector AOSLO system.
- Compared the Dove prism approach with a standard two-detector configuration.
- Utilized image combination techniques previously developed for four-detector systems.
Main Results:
- The Dove prism enabled resolution of retinal structures at multiple orientations.
- The new method successfully enhanced contrast for structures previously difficult to visualize.
- Combined images demonstrated improved visualization of retinal topography.
Conclusions:
- Rotating the light path with a Dove prism is an effective strategy to improve split-detection imaging in AOSLO.
- This technique expands the applicability of phase contrast imaging for retinal structure analysis.
- The method offers a viable alternative to more complex optical setups for enhanced retinal imaging.
