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Genetic Screens

Genetic Screens

Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which result in visible changes...

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Related Experiment Video

Updated: Jul 17, 2026

Genetic Profiling and Genome-Scale Dropout Screening to Identify Therapeutic Targets in Mouse Models of Malignant Peripheral Nerve Sheath Tumor

Genetic Profiling and Genome-Scale Dropout Screening to Identify Therapeutic Targets in Mouse Models of Malignant Peripheral Nerve Sheath Tumor

Published on: August 25, 2023

Functional allelic diversity of PTGS components revealed by a high-throughput RUBY-based screening.

Xin Wei^1,2, Yanming Hou², Yuyang Si²

¹Northeast Forestry University, Northeast Asia Biodiversity Research Center, Harbin, 150040, China.

The Plant Journal : for Cell and Molecular Biology

|July 16, 2026

Summary

This study identifies new mutations in genes controlling post-transcriptional gene silencing (PTGS). These findings offer valuable genetic tools for understanding PTGS mechanisms and improving gene expression control.

Keywords:

PTGS component alleles RNA decay RUBY forward genetic screening protein structure prediction small RNA

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Last Updated: Jul 17, 2026

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Published on: May 25, 2015

Area of Science:

Molecular Biology
Genetics
Plant Science

Background:

Post-transcriptional gene silencing (PTGS) regulates gene expression via small RNAs, impacting transgene and endogenous gene expression.
Limited characterized mutations in PTGS components hinder mechanistic understanding and domain function studies.

Purpose of the Study:

To identify novel mutations in PTGS components using a high-throughput screening approach.
To investigate the functional roles of specific protein domains within PTGS factors.

Main Methods:

Combined the ein5-1 RNA decay mutant with the RUBY reporter system for high-throughput screening.
Identified 239 mutations across key PTGS components and novel factors.
Performed systematic phenotypic evaluation of identified mutants.

Main Results:

Discovered mutations affecting critical protein domains in PTGS components.
Observed spatial-temporal repression of PTGS in various mutants.
Found that mutations in catalytic sites, RNA-binding sites, or interaction interfaces reduced siRNA production.

Conclusions:

Generated valuable mutant alleles for studying domain-specific functions of PTGS factors.
These resources aid in understanding PTGS mechanisms and offer strategies for fine-tuning gene silencing efficiency.