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Updated: Aug 5, 2026

Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro
Published on: April 8, 2020
Stabilization of Enzymes Through Encapsulation in Liposomes
1Department of Applied Chemistry, Yamaguchi University, Tokiwadai 2-16-1, Ube, 755-8611, Japan. yosimoto@yamaguchi-u.ac.jp.
Abstract:
Phospholipid vesicle (liposome) offers an aqueous compartment surrounded by lipid bilayer membranes. Various enzyme molecules were reported to be encapsulated in liposomes. The liposomal enzyme shows peculiar catalytic activity and selectivity to the substrate in the bulk liquid, which are predominantly derived from the substrate permeation resistance through the membrane. We reported that the quaternary structure of bovine liver catalase and alcohol dehydrogenase was stabilized in liposomes through their interaction with lipid membranes. The method and condition for preparing the enzyme-containing liposomes with well-defined size, lipid composition and enzyme content are of particular importance, because these properties dominate the catalytic performance and stability of the liposomal enzymes.
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