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FAD-Dependent Glucose Dehydrogenase Immobilization and Mediation Within a Naphthoquinone Redox Polymer
1Milton Research Group, Department of Inorganic and Analytical Chemistry, Faculty of Sciences, University of Geneva, Geneva 4, Switzerland. Ross.Milton@unige.ch.
Abstract:
Electrochemically active polymers (redox polymers) are useful tools for the simultaneous immobilization and electron transfer of enzymes at electrode surfaces, which also serve to increase the localized concentration of the biocatalyst. The properties of the employed redox couple must be compatible with the target biocatalyst from both an electrochemical (potential) and biochemical standpoint. This chapter details the synthesis of a naphthoquinone-functionalized redox polymer (NQ-LPEI) that is able to immobilize and electronically communicate with flavin adenine dinucleotide-dependent glucose dehydrogenase (FAD-GDH), yielding an enzymatic bioanode that is able to deliver large catalytic current densities for glucose oxidation with a relatively low associated onset potential of catalysis. This redox polymer has also enabled mediated electron transfer with additional enzymes as well as intact microbes.
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