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Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures
Published on: January 7, 2019
Overlooked properties difference of biogenic sulfur resulting in distinct performance in serving as an electron donor
1State Key Laboratory of Urban-rural Water Resources and Environment, School of Eco-Environment, Harbin Institute of Technology Shenzhen, Shenzhen, 518055, PR China.
Abstract:
Biogenic sulfur (bio-S0) is recognized as a more efficient electron donor compared to its chemical counterpart in microbial denitrification. However, the performance differences among various types of bio-S0 remain poorly understood, posing a challenge in identifying the optimal variant. In this study, we comparatively investigated three distinct bio-S0 samples-bio-S0-A1 and bio-S0-A2, both obtained from single-reactor bio-S0 production processes with varying organic coating thicknesses, and bio-S0-B, sourced from a dual-reactor bio-S0 production process-alongside chemical sulfur (chem-S0) in terms of property characterization, denitrification performance, and microbial composition. Our results showed that bio-S0-B exhibited an elevated denitrification rate compared to the chem-S0, but was less effective than bio-S0-A1 and bio-S0-A2. This discrepancy could be attributed to the differing sulfur-related properties, such as polysulfane content and crystallinity. Regarding the other two bio-S0 samples (bio-S0-A1 and bio-S0-A2), our findings indicated that the organic coating negatively impacted denitrification performance. Inspired by this, we further demonstrated that bio-S0 can be optimized by destroying the organic coating, leading to an increase of up to 3.2-fold in denitrification rate. Variations of microbial composition were noted among different bio-S0 samples, with the relative abundance of Moheibacter showing a positive correlation with the denitrification rate. This study offers a property-based guideline for optimizing the selection of bio-S0 and further refining raw bio-S0 to enhance its effectiveness as an electron donor for denitrification.
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