Injectable Thermoresponsive PIB/PDA/Doxycycline Hydrogels with Antibacterial, Antioxidant, and Temperature-Triggered
Yuanjing Zhang1, Zheng Chen2,3, Jiaxuan Guo3
1Department of Dermatology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, , University of Science and Technology of China, Hefei, Anhui Province230026, P. R. China.
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Injectable and thermoresponsive hydrogels integrating hemostasis, infection suppression, and oxidative stress mitigation are highly desirable for complex wound management and intravascular intervention scenarios. Herein, we developed a biocompatible and thermoresponsive hydrogel based on a poly(N-isopropylacrylamide-co-butyl methacrylate) (PIB) matrix modified with polydopamine (PDA) and loaded with doxycycline (PIB/PDA/Doxycycline). PDA endowed efficient 808 nm photothermal conversion with robust cycling stability, as well as broad-spectrum radical scavenging activity. The hydrogel enables controlled and sustained release of doxycycline over 300 h, and in vitro assays confirmed synergistic chemo-photothermal antibacterial activity against Staphylococcus aureus and Escherichia coli. In a murine full-thickness wound model, the PIB/PDA/Doxycycline achieved nearly complete wound closure with improved tissue regeneration. In a murine tail-transection bleeding model, the hydrogel rapidly formed a physical gel barrier at 37 °C, leading to a significant reduction in blood loss. This integrated hydrogel system holds promise for wound repair, antibacterial treatment, antioxidant microenvironment regulation, and temperature-triggered hemostatic applications.
