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[Retracted] Matrine‑induced apoptosis in Hep3B cells via the inhibition of MDM2
Ning Zhou1, Jiequn Li1, Ting Li1
1Department of Organ Transplantation and General Surgery, The Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, P.R. China.
Abstract:
Following the publication of the above paper, the following areas of concern associated with the western blot data featured in Figs. 3, 4 and 5 were drawn to the Editor's attention by a concerned reader, essentially involving the apparent incorrect assembly of blots within these figures and duplications of data: i) Control blots were shared between Figs. 3A and 5A, where the experimental conditions were reported to be different; ii) MDM2 blots may have been featured incorrectly in Figs. 4B and 5A, as the control blots/reported experimental conditions featured in these figure parts appeared to be the same; and iii) there was also the probable duplication of protein bands of interest comparing Fig. 5A and B. A subsequent analysis of the data in this paper performed by the Editorial Office also revealed the potential re‑use of data in a paper that was published a year later, also in the journal Molecular Medicine Reports, albeit no authors were held in common comparing these papers, and that paper has since been retracted on account of the re‑use of data with another article: Given the identification of the above problems associated with the assembly of data in three of the figures in this paper, the Editor has decided that it should be retracted from the Journal on account of a lack of confidence in the data. The authors were asked for an explanation to account for these concerns, but the Editorial Office did not receive a reply. The Editor apologizes to the readership for any inconvenience caused. [Molecular Medicine Reports 15: 442‑450, 2017; DOI: 10.3892/mmr.2016.5999].
Insights
Concerns regarding western blot data assembly and duplication led to the retraction of this study. A lack of confidence in the presented data prompted the journal editor to withdraw the publication.
Area of Science:
- Molecular Biology
- Biochemistry
- Scientific Publishing Ethics
Background:
- Concerns were raised regarding the integrity of western blot data presented in Figures 3, 4, and 5.
- Specific issues included apparent incorrect assembly of blots and data duplication across different experimental conditions.
Purpose of the Study:
- To address reader-reported concerns about the validity of western blot data.
- To investigate potential data duplication and misrepresentation in the published figures.
Main Methods:
- Editor's review of western blot figures (3, 4, and 5).
- Analysis of data for inconsistencies in control blots and experimental conditions.
- Investigation of potential data reuse with another publication.
Main Results:
- Identified shared control blots between figures with differing experimental conditions.
- Noted potential misrepresentation of MDM2 blots due to similar control data.
- Discovered probable duplication of protein bands between figures.
- Confirmed potential data reuse with a subsequently retracted article.
Conclusions:
- The identified data assembly issues and potential duplication led to a lack of confidence in the study's findings.
- The journal editor decided to retract the paper due to concerns about data integrity.
- Authors did not provide a satisfactory explanation for the discrepancies.
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