[Retracted] Matrine‑induced apoptosis in Hep3B cells via the inhibition of MDM2

Ning Zhou1, Jiequn Li1, Ting Li1

  • 1Department of Organ Transplantation and General Surgery, The Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, P.R. China.

Insights

Concerns regarding western blot data assembly and duplication led to the retraction of this study. A lack of confidence in the presented data prompted the journal editor to withdraw the publication.

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Scientific Publishing Ethics

Background:

  • Concerns were raised regarding the integrity of western blot data presented in Figures 3, 4, and 5.
  • Specific issues included apparent incorrect assembly of blots and data duplication across different experimental conditions.

Purpose of the Study:

  • To address reader-reported concerns about the validity of western blot data.
  • To investigate potential data duplication and misrepresentation in the published figures.

Main Methods:

  • Editor's review of western blot figures (3, 4, and 5).
  • Analysis of data for inconsistencies in control blots and experimental conditions.
  • Investigation of potential data reuse with another publication.

Main Results:

  • Identified shared control blots between figures with differing experimental conditions.
  • Noted potential misrepresentation of MDM2 blots due to similar control data.
  • Discovered probable duplication of protein bands between figures.
  • Confirmed potential data reuse with a subsequently retracted article.

Conclusions:

  • The identified data assembly issues and potential duplication led to a lack of confidence in the study's findings.
  • The journal editor decided to retract the paper due to concerns about data integrity.
  • Authors did not provide a satisfactory explanation for the discrepancies.