Related Experiment Video
Updated: Jul 30, 2026

An Optimized Protocol to Analyze Glycolysis and Mitochondrial Respiration in Lymphocytes
Published on: November 21, 2016
Glycolytic control of respiration during aging of carrot root tissue
Abstract:
Changes in respiration in aging carrot slices are correlated with a characteristic sequence of activation of the irreversible reactions of the glycolytic pathway. The induced "wound" respiration increases in two stages; these appear to correspond to a period of active synthesis, when ADP, produced in the cytoplasm from synthetic reactions, activates pyruvate kinase, and a period of readjustment when ATP, no longer reacting in synthetic reactions, activates phosphofructokinase. Although ATP is cited frequently as a negative effector of phosphofructokinase, unless the concentration of ATP at the site of phosphofructokinase is 50-fold higher than the concentration expressed per unit fresh weight of the tissue, it cannot act in this way in carrot slices. The activation of the oxidative pentose phosphate pathway observed by ap Rees and Beevers is restricted to the first stage of the induced respiration.
Related Concept Videos
What is Glycolysis?
Cells make energy by breaking down macromolecules. Cellular respiration is the biochemical process that converts "food energy" (from the chemical bonds of macromolecules) into chemical energy in the form of adenosine triphosphate (ATP). The first step of this tightly regulated and intricate process is glycolysis. The word glycolysis originates from the Latin glyco (sugar) and lysis (breakdown). Glycolysis serves two main intracellular functions: generating ATP and generating...
Energy-releasing Steps of Glycolysis
The first energy-releasing step—the 6th step of glycolysis —consists of two...
Outcomes of Glycolysis
Cellular respiration can occur aerobically (with oxygen) or anaerobically (without oxygen). In the presence of oxygen, cellular respiration starts with glycolysis and continues with pyruvate oxidation, the...
Mitochondria
Fates of Pyruvate
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...

