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Construction of a Modified Pain-Induced Stress White Hair Model
Xunhong Xu1,2,3, Shenhua Wu2,3, Yarui Zhang2,3
1School of Public Health, Southern Medical University, Guangzhou, China.
Experimental Dermatology
|July 17, 2026
Summary
Resiniferatoxin (RTX) effectively models pain-induced white hair. A 90 μg/kg dose in 4-week-old male mice, combined with depilation, creates a stable premature greying model with reduced melanin.
Area of Science:
- Toxicology
- Dermatology
- Animal Models
Background:
- Resiniferatoxin (RTX) is used to model pain-induced white hair.
- Optimal dosage, injection, and localization for RTX-induced greying are unclear.
Purpose of the Study:
- Establish a stable mouse model for pain-induced stress-associated premature greying.
- Investigate RTX application parameters including sex, age, dose, depilation, and injection method.
Main Methods:
- Multidimensional approach analyzing RTX effects on white hair.
- Histological and immunofluorescence analyses of melanin granules and TRP2-positive cells.
- Open-field tests and weight monitoring to assess stress response.
Main Results:
- 90 μg/kg RTX induced the largest stable white hair area without mortality.
- Male mice and 4-week-old males showed higher white hair proportions.
- Reduced melanin granules and TRP2-positive cells observed in white hair follicles.
Conclusions:
- A stable model of pain-induced hair depigmentation was established in 4-week-old male mice using RTX.
- Intraperitoneal RTX injection at 90 μg/kg with depilation accelerated white hair formation.
- The model demonstrates reduced melanin in hair follicles, correlating with stress-induced greying.
