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Updated: Aug 6, 2026
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Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing
Published on: September 25, 2011
Fluorescent Anti-CEA Antibody Detects Submillimeter Colon Cancer Metastases
Javier Bravo1, Shanglei Liu1, Sunidhi Jaiswal1
1Department of Surgery, University of California San Diego, San Diego, California; VA San Diego Healthcare System, San Diego, California.
The Journal of Surgical Research
|July 17, 2026
Summary
SGM-101 antibody imaging successfully detected small colon cancer micrometastases in preclinical and clinical models. This near-infrared fluorophore shows potential for future tumor staging and fluorescence-guided surgery in colorectal cancer (CRC) patients.
Area of Science:
- Oncology
- Medical Imaging
- Biotechnology
Background:
- Carcinoembryonic antigen (CEA) targeted SGM-101 is a near-infrared fluorophore for colorectal cancer (CRC) imaging.
- Previous studies indicated SGM-101's promise in CRC detection.
Purpose of the Study:
- To determine if SGM-101 fluorescence colocalizes with tumor luciferase expression.
- To assess SGM-101's ability to detect colon cancer micrometastases using preclinical and clinical imaging systems.
Main Methods:
- Orthotopic and subcutaneous human CRC cell xenografts were established in nude mice.
- Mice received SGM-101 and D-luciferin; imaging was performed using Pearl and Arthrex Synergy Vision systems.
- Tumor-to-background ratios (TBRs) were calculated to quantify SGM-101 labeling.
Main Results:
- SGM-101 fluorescence colocalized with luciferase expression in both tumor models.
- High TBRs were observed in orthotopic tumors (5.24 ±0.93).
- Microscopic (<1 mm) peritoneal metastases were detected by both imaging systems.
Conclusions:
- SGM-101 enables high-contrast tumor detection and colocalizes with luciferase expression in CRC models.
- SGM-101 successfully detected colon cancer micrometastases.
- Findings support SGM-101's clinical potential for tumor staging and fluorescence-guided surgery.
