Related Experiment Video
Updated: Aug 6, 2026

A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica
Published on: February 20, 2012
Genetic diversity and phylogenetic relationships in white lupin
Paolo Annicchiarico1, Bruno Nevado2,3, Nelson Nazzicari4
1Council for Agricultural Research and Economics (CREA), Research Centre for Animal Production and Aquaculture, Viale Piacenza 29, Lodi, 26900, Italy. paolo.annicchiarico@crea.gov.it.
Background:
Information on the genetic variation and phylogenetic relationships of white lupin (Lupinus albus L.) can support the increasing plant breeding effort on this crop. This study aimed to: (a) assess the genetic variation relative to germplasm type (landrace, modern cultivar or wild type), landrace region of origin, landrace accession within region, and genotype within landrace accession; (b) explore landrace phylogenetic relationships; and (c) assess the consistency between molecular and morphophysiological diversity.
Results:
A landrace collection of 112 accessions classified into 11 regional pools and a set of modern cultivars underwent genotyping-by-sequencing characterization (genotyping four plants per landrace) and evaluation of 11 morphophysiological traits. Molecular data of four wild-type accessions were added. Multi-dimensional scaling indicated a sharp genetic distinctness of wild-type material and a fairly modest diversity of modern cultivars relative to landraces. Mantel's test revealed low consistency between molecular and morphophysiological diversity. Overall, landrace germplasm exhibited high genetic differentiation. The size of variance components in an analysis of molecular variance ranked as: genotype within landrace > landrace within regional pool > regional pool. Cluster analysis, nucleotide diversity, number of private alleles, phylogeny and migration analyses suggested a dual or divergent crop domestication in Greece and Italy, followed by migration of Greek germplasm into North-Eastern Africa and the Near East, extensive migration of Italian germplasm in the Mediterranean basin, and hybridization of the two genetic pools in various regions.
Conclusions:
Our results increase our knowledge of white lupin diversity and phylogeny and can help optimize the exploitation of genetic resources.
Related Concept Videos
Evolutionary Relationships through Genome Comparisons
Dihybrid Crosses
Trihybrid Crosses
Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
The F1 generation plants of a trihybrid cross are heterozygous for all three traits and produce eight gametes. Upon self-fertilization, these gametes have an equal chance to...
Incomplete Dominance
Monohybrid Crosses
Gene Evolution - Fast or Slow?
In contrast, regions which code...
