Development and application of whole genome SNP markers in mango

Area of Science:

• Plant genetics
• Molecular biology
• Agricultural science

Background:

• Mango (Mangifera indica L.) is a globally important fruit crop.
• Accurate identification of mango germplasm is crucial for developing new cultivars.
• Traditional identification methods are labor-intensive and lack precision, necessitating advanced techniques.

Purpose of the Study:

• To develop and validate single nucleotide polymorphism (SNP) markers for mango germplasm identification.
• To construct DNA fingerprints for mango germplasm resources.
• To facilitate genetic diversity analysis and support molecular marker-assisted breeding in mango.

Main Methods:

• Genomic-wide SNP discovery and filtering using GATK software.
• Development of 85 novel PARMS-SNP markers from high-quality SNP sites.
• Application of selected SNP markers for DNA fingerprinting and genetic grouping of 145 mango accessions.

Main Results:

• Identified 272 high-quality SNP sites and developed 85 novel PARMS-SNP markers.
• Successfully grouped 145 mango germplasm accessions into three major categories.
• Constructed unique DNA fingerprints for each accession, confirming accurate identification and enabling parent-offspring relationship determination.

Conclusions:

• This study represents a pioneering effort in establishing genome-wide SNP markers for mango.
• The developed SNP markers provide a robust foundation for mango germplasm utilization and molecular breeding.
• The findings enable precise identification of mango varieties and efficient breeding strategies.