EID1 overexpression in brown adipose tissue enhances cold-induced thermogenesis in male mice

E1A-like inhibitor of differentiation 1 (EID1), a nuclear coregulator, has been implicated in adipocyte differentiation and lipid metabolism. In vitro, EID1 overexpression suppresses lipid accumulation in preadipocytes; however, its role in thermogenesis in vivo remains unclear. We generated adipose tissue-specific EID1 transgenic (Tg) mice and assessed their thermogenic capacity under cold exposure. During cold exposure, male Tg mice exhibited more than a 7-fold increase in interscapular brown adipose tissue (BAT) glucose uptake, nearly triple the response observed in wild-type (Wt) mice. Core body temperature was better maintained in Tg mice, with attenuated cold-induced decline. Infrared thermography revealed a 5-fold greater increase in BAT temperature in Tg mice than in the Wt controls. These responses were accompanied by robust upregulation of thermogenesis-related genes. These findings highlight EID1 as a dynamic regulator of thermogenic capacity and suggest that modulating its stability and activity may represent a new strategy to improve metabolic health.