SemProtDB: A Reference Database of Seminal Plasma Proteins

Seminal plasma proteins are vital for sperm metabolism, protection against oxidative stress, fertility, and early embryo development. A substantial body of data evidence that seminal plasma proteomes are highly species-specific. However, this information remains dispersed across the literature, hindering comparative analyses and limiting the identification of potential, conserved fertility-related biomarkers. To address this gap, we developed SemProtDB (https://semprotdb.org/), the first centralized database of seminal plasma from domestic mammals. It consolidates data from 28 studies, featuring nearly 18,000 protein entries from 8 major species: Bos taurus, Bubalus bubalis, Capra hircus, Ovis aries, Equus caballus, Equus asinus, Canis lupus familiaris, and Sus scrofa. With more than 1500 proteins manually curated, the database provides a user-friendly interface linked to UniProt, NCBI, STRING, and source publications. SemProtDB represents an unprecedented resource for reproductive biology, facilitating comparative and translational research to better understand the molecular mechanisms underlying fertility across species.