Related Experiment Video
Updated: Aug 6, 2026
09:09
Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics
Published on: October 13, 2020
In vitro peptidoglycan degradation assay using fluorescein-5-isothiocyanate-labelled sacculi
Dema Alodaini1, Adam Hart2, Patrick J Moynihan1
1School of Biosciences, University of Birmingham, Birmingham, UK.
Access Microbiology
|July 20, 2026
Abstract:
We describe a method using fluorescein-5-isothiocyanate (FITC)-labelled peptidoglycan sacculi to characterize peptidoglycan hydrolases in vitro. In this protocol, soluble hydrolytic products released by peptidoglycan hydrolases are separated from the insoluble FITC-sacculi, quantified and set in relation to the overall available substrate. This enables the study of hydrolase activity in end-point or time-course experiments.
