Advances in cell-derived extracellular matrix for 3D tumor modelling

Area of Science:

• Biomaterials Science
• Tissue Engineering
• Cancer Research

Background:

• Cell-derived decellularized extracellular matrix (cdECM) is a promising biomaterial for creating 3D tumor models.
• cdECM can mimic the tumor microenvironment's biomolecular diversity using human cells, offering an advantage over non-human materials.

Purpose of the Study:

• To review strategies for modulating human ECM production in vitro.
• To showcase advances in cdECM decellularization and processing for bioengineered tumor models.
• To discuss the biological relevance and challenges of using cdECM in preclinical models.

Main Methods:

• Review of established and emerging methods for in vitro ECM modulation.
• Analysis of decellularization and processing techniques across various scales and complexities.
• Discussion of cdECM incorporation into bioengineered tumor model design.

Main Results:

• Human cdECM provides a biomimetic and tumor-specific scaffold.
• Diverse decellularization and processing methods exist for cdECM.
• cdECM integration enhances the biological relevance of engineered tumor models.

Conclusions:

• cdECM is a valuable biomaterial for developing advanced 3D tumor models.
• Further innovation in cdECM production and processing is needed for preclinical applications.
• Cell-derived biomaterials hold significant potential for tumor modeling and broader tissue engineering.