Comparing the volume of umbilical cord blood collection using different techniques

Area of Science:

• Neonatal Medicine
• Hematology
• Biotechnology

Background:

• Umbilical cord blood (UCB) is a promising source for red blood cell (RBC) transfusions in extremely preterm neonates due to its high fetal hemoglobin (HbF) content.
• Current limitations in UCB use include low and variable collection yields.
• This study evaluated UCB collection techniques in term pregnancies to address yield variability.

Purpose of the Study:

• To assess and compare the volume and feasibility of UCB collections using three distinct techniques.
• To determine the optimal technique for maximizing UCB yield for potential transfusion applications.
• To identify factors influencing UCB collection volume.

Main Methods:

• A prospective observational study was conducted in two Dutch centers.
• Three UCB collection techniques were compared: in utero, ex utero with a funnel, and ex utero with a plateau.
• A minimum target volume of 70 mL was set as adequate for processing.

Main Results:

• Out of 606 collection attempts, 570 (94%) were successful, with a median UCB volume of 65 mL.
• The ex utero plateau technique yielded the highest median volume (70 mL), significantly more than the in utero (56 mL) and funnel (36 mL) techniques.
• UCB volume correlated positively with birth weight and showed a learning curve effect for the plateau technique.

Conclusions:

• The ex utero plateau technique is the most effective for maximizing UCB collection volume.
• Birth weight and operator experience are key factors influencing UCB yield.
• Dedicated teams and continuous training are essential for improving UCB collection efficiency for transfusion purposes.