Related Experiment Video
Updated: Aug 6, 2026
Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
Published on: December 23, 2022
Draft genome sequence of Enterobacter roggenkampii strain MBBL_MP5 isolated from dairy cow with clinical mastitis
Kh Yeashir Arafat1, Md Morshedur Rahman1, Md Anamul Kabir Hemal1
1Molecular Biology and Bioinformatics Laboratory, Department of Gynecology, Obstetrics and Reproductive Health, Gazipur Agricultural University, Gazipur, Bangladesh.
Abstract:
We report the draft genome of Enterobacter roggenkampii MBBL_MP5 isolated from milk of a dairy cow with clinical mastitis. The ~4.7 Mbp genome of MBBL_MP5 encodes 21 antimicrobial resistance genes (ARGs) and 87 virulence factor genes (VFGs), highlighting its possible role in bovine mastitis and relevance to public health.
Insights
We sequenced the genome of *Enterobacter roggenkampii* MBBL_MP5, found in cow mastitis milk. This bacterium possesses numerous antimicrobial resistance and virulence genes, indicating its potential impact on animal and public health.
Area of Science:
- Microbiology
- Genomics
- Veterinary Medicine
Background:
- Clinical mastitis is a significant concern in dairy herds, impacting animal health and milk production.
- Understanding the genetic basis of bacterial pathogens involved in mastitis is crucial for effective treatment and control.
- Enterobacter species are increasingly recognized as opportunistic pathogens in both humans and animals.
Purpose of the Study:
- To perform whole-genome sequencing of *Enterobacter roggenkampii* MBBL_MP5, an isolate from bovine clinical mastitis.
- To identify antimicrobial resistance genes (ARGs) and virulence factor genes (VFGs) within the MBBL_MP5 genome.
- To assess the potential role of this isolate in bovine mastitis and its public health implications.
Main Methods:
- Whole-genome sequencing of *Enterobacter roggenkampii* MBBL_MP5.
- Bioinformatic analysis to identify ARGs and VFGs.
- Comparative genomics to understand potential pathogenicity.
Main Results:
- The draft genome size of *Enterobacter roggenkampii* MBBL_MP5 is approximately 4.7 Mbp.
- The genome encodes 21 antimicrobial resistance genes (ARGs).
- The genome encodes 87 virulence factor genes (VFGs).
Conclusions:
- The genetic makeup of *Enterobacter roggenkampii* MBBL_MP5, including its ARGs and VFGs, suggests a significant role in bovine mastitis.
- This finding underscores the importance of genomic surveillance of mastitis-causing bacteria for public health.
- Further research is warranted to explore the transmission dynamics and pathogenicity mechanisms of this strain.
