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Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry
Published on: March 5, 2019
An optimized three-laser 27-color spectral flow cytometry panel for multi-organ profiling in mice
Hyeonji Song1, Yusik Lim2, Jaechul Lim1,3
1College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea.
Plos One
|July 20, 2026
Summary
Researchers developed a 27-color flow cytometry panel for profiling 16 mouse cell types, addressing reagent scarcity and spectral overlap challenges. This tool aids in dissecting complex cellular landscapes in murine models.
Area of Science:
- Immunology
- Cell Biology
- Biotechnology
Background:
- High-dimensional flow cytometry is crucial for cellular network analysis.
- A lack of comprehensive mouse cell panels hinders research due to spectral overlap and reagent limitations.
Purpose of the Study:
- To develop and validate a 27-color flow cytometry panel for simultaneous profiling of diverse mouse cell types.
- To overcome technical challenges in murine immune cell analysis.
Main Methods:
- Designed a 27-color flow cytometry panel optimized for 3-laser spectral cytometers.
- Validated the panel across multiple mouse tissues and in a poly(I:C) model.
Main Results:
- Enabled simultaneous detection of 16 distinct lymphoid, myeloid, and non-immune cell subsets.
- Successfully tracked immune cell population shifts in vivo.
- Identified a novel Cyp11b1-expressing immune cell subset.
Conclusions:
- The developed panel facilitates high-dimensional immune profiling on standard 3-laser cytometers.
- Provides a robust tool for dissecting cellular landscapes in diverse murine contexts.
