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MicroRNA-423-5p Inhibition is Associated with Reduced Infarct Severity After Permanent Cerebral Ischemia in Rats
Xue Yang1, Junfang Yang2, Tao Zang3
1Department of Neurology, Fujian Provincial Governmental Hospital.
Journal of Visualized Experiments : Jove
|July 20, 2026
Summary
Inhibiting microRNA-423-5p in a rat model of cerebral infarction reduced neurological deficits and infarct size. This suggests microRNA-423-5p plays a role in ischemic brain injury progression.
Area of Science:
- Neuroscience
- Molecular Biology
- Genetics
Background:
- Acute cerebral infarction leads to irreversible injury and secondary damage.
- Apoptosis-related signaling pathways are implicated in the progression of ischemic brain lesions.
Purpose of the Study:
- To investigate the association between microRNA-423-5p inhibition and early outcomes in a rat model of permanent middle cerebral artery occlusion.
- To explore the role of microRNA-423-5p in cerebral ischemia and apoptosis.
Main Methods:
- Adult male Sprague-Dawley rats underwent permanent middle cerebral artery occlusion.
- microRNA-423-5p was inhibited using an interfering adenovirus.
- Neurological deficits, infarct volume, and protein expression (Bax, Bcl-2) were assessed.
Main Results:
- microRNA-423-5p inhibition correlated with lower neurological deficit scores and reduced infarct volume.
- Inhibition led to decreased Bax expression and increased Bcl-2 expression.
- Reduced microRNA-423-5p abundance was observed in ischemic brain tissue.
Conclusions:
- Inhibition of microRNA-423-5p shows a preliminary association with reduced injury severity in permanent cerebral ischemia.
- Further studies are needed to validate direct targets and apoptosis assays to elucidate the mechanism.