Murine Model for Selective Transection of Intestinal Autonomic Nerves

Autonomic nerves comprise the sympathetic and parasympathetic systems, which act in a largely antagonistic manner to regulate diverse physiological functions. With growing interest in the roles of autonomic innervation in intestinal physiology and pathology, there is an increasing need for experimental models that enable precise manipulation of gut-directed sympathetic and parasympathetic inputs. Here, we present a surgical protocol to selectively transect the sympathetic and parasympathetic nerves innervating the intestine. Using the superior mesenteric artery (SMA) as a reproducible anatomical landmark, this approach allows reliable identification of intestinal autonomic nerve bundles and subsequent targeted transection. In contrast to broader denervation approaches such as ganglionectomy or subdiaphragmatic vagotomy, this approach is intended to achieve a more anatomically localized transection of intestine-directed autonomic bundles, which may help reduce extra-intestinal interference. The injury is controllable and is typically associated with minimal bleeding, supporting procedural consistency and postoperative recovery. Although the surgery requires appropriate microsurgical training, the protocol is adaptable, enabling users to tailor key parameters to specific experimental aims. Importantly, this model provides a practical platform to investigate the roles of sympathetic and parasympathetic nerves in regulating intestinal function under both physiological and disease conditions. This article aims to describe the procedure in detail to facilitate its adoption by new users in their research.