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Updated: Aug 6, 2026

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures
Published on: January 7, 2019
Carbon source-driven anaerobic assimilation of dye-derived dissolved organic nitrogen: A low-carbon strategy with
Xumeng Lin1, Huahan Huang1, Ying Wu1
1College of Environmental Science and Engineering, Donghua University, Shanghai, 201620, PR China.
Abstract:
This study demonstrates that supplementing external carbon sources enables a sustainable anaerobic strategy for removing dye-derived dissolved organic nitrogen (DON). At a chemical oxygen demand/total nitrogen (COD/TN) ratio of 40 and an 8 h hydraulic retention time, a glucose-fed system achieved superior removal efficiencies (85.72% TN, 94.71% COD) compared to those of an acetate-fed system (51.68% TN, 90.20% COD). Mass balance showed that glucose facilitated greater assimilation of TN (85.65%) and total organic carbon (91.20%) into biomass than acetate (51.71% and 88.10%, respectively). Amino acid content increased by 44.17% in the glucose system versus 25.43% in the acetate system. Functional consortia (e.g., norank_Thermodesulfovibrionia, norank_Anaerolineae) were enriched in the glucose-fed system. Mechanistically, glucose metabolism activated the Embden-Meyerhof-Parnas pathway and the reductive tricarboxylic acid cycle, enhancing the supply of precursors (e.g., pyruvate) and energy (ATP, NADH) for protein biosynthesis, whereas acetate metabolism was limited in these aspects. These findings reveal a potential low-carbon DON removal mechanism that captures nitrogen into microbial protein, bypassing the energy-intensive gasification steps required in conventional treatment processes.
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