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PpPRE1 and PpPRE2 mediate gibberellin-induced internode elongation by regulating brassinosteroid biosynthesis in
Huanbing Lu1, Mengmeng Zhang2, Ning Liu1
1College of Horticulture, Henan Agricultural University, 95 Wenhua Road, Zhengzhou 450002, China; International Joint Laboratory of Henan Horticultural Crop Biology, Zhengzhou 450046, China.
None:
Controlling shoot elongation is critical for peach orchard management. Paclobutrazol resistance (PRE) play crucial roles in promoting cell elongation and plant growth. In this study, we found that peach PRE1 and PRE2 respond to GA signaling and promote internode elongation by enhancing BR biosynthesis. Peach has four PRE genes, all of which exhibit higher expression levels in young tissues compared to mature tissues. Analysis of expression and internode length indicated that PpPRE1 and PpPRE2 are highly expressed in actively elongating internodes. Further investigation revealed that silencing of PpPRE1 and PpPRE2 downregulated the expression levels of BR-biosynthesis-related genes, leading to reduced BR content and a significant decrease in internode elongation. Furthermore, we found that the BR biosynthesis inhibitor BRZ markedly inhibited GA-promoted shoot elongation in peach. In GA-insensitive peach shoots, the expression of PpPRE1 and PpPRE2 was significantly reduced, whereas exogenous GA application upregulated their expression. Meanwhile, knockdown of these two genes also substantially attenuated the promotion of GA on elongation. This indicates that PpPRE1 and PpPRE2 mediate the promotion of GA on internode elongation. All together, these results demonstrate that PpPRE1 and PpPRE2 are key genes in the regulatory network governing internode elongation, providing potential targets for breeding new peach varieties with shortened internodes.
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